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Sensory Stimuli Directly Acting at the Central Nervous System Regulate Gastric Ghrelin Secretion. An ex Vivo Organ Culture Study

Endocrinología Molecular (L.M.S., O.A.-M., F.F.C.), Area de Investigacion, Complejo Hospitalario Universitario de Santiago (CHUS), E-15780, Santiago de Compostela, Spain; Departaments of Medicine (O.A.-M., F.F.C.) and Physiology (J.E.C., S.A.T., C.D.), Santiago de Compostela University, 15782 Santiago de Compostela, Spain; Centro de Investigaciones Biomédicas en Red (CIBER): Fisiopatología de la obesidad y nutrición (CB06/03) (L.M.S., C.D., F.F.C.), Instituto de Salud Carlos III, Spain; and Department of Physiology (J.E.C.), School of Medicine, National University of Colombia, 1101 Bogotá, Colombia

Address all correspondence and requests for reprints to: Luisa M. Seoane, Area de Investigacion, Complejo Hospitalario Universitario de Santiago de Compostela, P.O. Box 563, E-15780, Santiago de Compostela, Spain. E-mail: fssisisc{at}usc.es.

Ghrelin, a novel gastrointestinal hormone involved in GH regulation, has been postulated as a relevant orexigenic peptide released by splanchnic tissues. Descriptive studies have shown that plasma ghrelin levels increase in states of negative energy balance or fasting, while decreasing in obesity and after feeding. In the present study, a novel organ-culture model of gastric tissue explants obtained from rat donors has been validated for ex vivo experiments. Fasting induced gastric ghrelin release as well as ghrelin mRNA expression that were reflected in plasma. Interestingly, those changes were fully reverted by 15 min of refeeding before stomach extraction. Unexpectedly, when animals were allowed 15 min before explant extraction to see or smell, but not eat, the food (tease feeding), ghrelin secretion was suppressed just like in gastric explants from refed animals. This effect was blocked when the animals were subjected to surgical vagotomy or treated with atropine sulphate. In conclusion, gastric explants were a suitable model for testing ghrelin mechanism of secretion in vitro, and they were found to maintain memory of the previously received signals. Similar to feeding, tease feeding resulted in suppression of ghrelin discharge by explants.