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This version published online on June 26, 2003
Endocrinology, doi:10.1210/en.2002-221001
A more recent version of this article appeared on October 1, 2003
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Submitted on September 25, 2002
Accepted on June 16, 2003

Molecular Evidence Of Organic Ion Transporters In The Rat Adrenal Cortex With Acth-Regulated Zonal Expression

E. Beéry1, P. Middel1, A. Bahn1, H. S. Willenberg1, Y. Hagos1, H. Koepsell1, S. R. Bornstein1, G. A. Müller1, G. Burckhardt1, and J. Steffgen1*

1 Abteilung Nephrologie und Rheumatologie, Abteilung Pathologie, Abteilung Vegetative Physiologie und PathophysiologieUniversität Göttingen, Abteilung Endokrinologie, Universität Düsseldorf, Anatomisches Institut, Lehrstuhl I, Universität Würzburg, Germany

* To whom correspondence should be addressed. E-mail: jsteffgen{at}gmx.de.

Experimental evidence suggested that secretion of steroid hormones from adrenocortical cells involves carrier-mediated transport: Cortisol release from, and uptake of p-[3H]aminohippurate (PAH) into, bovine adrenocortical cells showed properties of the renal PAH/anion exchanger OAT1. Other polyspecific transporters such as organic anion transporting polypeptides (oatps) and organic cation transporters (OCTs) could also be involved in steroid hormone release. A homology cloning procedure was established to detect these transporters in rat adrenal gland cDNA. PCR revealed the presence of OAT1, oatp1, oatp2 and oatp3. In situ hybridization localized OAT1 in the outer zona fasciculata, oatp3 in the zona glomerulosa, and oatp1 and oatp2 in the inner zona fasciculata and outer zona reticularis. An OCT2 specific probe produced signals in the zona glomerulosa and outer zona fasciculata. Pretreatment of rats with ACTH increased the expression of OAT1 mRNA which spread to all zones and hypophysectomy strongly decreased it. A less pronounced regulation was detected for OCT2 and oatp3. Specific antibodies confirmed the localization of OAT1 in the outer zona fasciculata, supporting a possible role of OAT1 in cortisol release. The zonated distribution of transporters furthermore suggest that oatp1-3 and OCT2 may be important for the endocrine function of rat adrenocortical cells.
Key words: OAT1 • oatp • OCT2 • adrenal cortex • corticosteroids




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