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This version published online on June 5, 2003
Endocrinology, doi:10.1210/en.2003-0314
A more recent version of this article appeared on September 1, 2003
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Submitted on March 11, 2003
Accepted on May 29, 2003

CaT1 and ECaC mRNA are Differentially Regulated by 1,25 dihydroxyvitamin D3 in the Intestine and Kidney of Mice

Yurong Song1, Xiaorong Peng1, Angela Porta1, Hitomi Takanaga1, Ji-Bin Peng1, Matthias A. Hediger1, James C. Fleet1*, and Sylvia Christakos1

1 Interdepartmental Nutrition Program, Purdue University, West Lafayette, IN 47907, Renal Division and Membrane Biology Program, Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, Dept. of Biochemistry and Molecular Biology, UMDNJ-New Jersey Medical School, Newark, NJ

* To whom correspondence should be addressed. E-mail: fleetj{at}cfs.purdue.edu.

We examined the expression of CaT1 and ECaC mRNA in the duodenum and kidney of mice. Intestinal CaT1 mRNA level increased 30-fold at weaning, coincident with the induction of calbindin-D9k expression. In contrast, renal CaT1 and ECaC mRNA expression was equal until weaning when ECaC mRNA is induced and CaT1 mRNA levels fall 70%. Long- and short-term adaptation to changes in dietary calcium (Ca) level and 1,25 dihyroxyvitamin D3 (1,25(OH)2 D3) injection strongly regulated duodenal calbindin D9k and CaT1 mRNA. Following a single dose of 1,25(OH)2 D3, induction of CaT1 mRNA occurred rapidly (within 3 h, peak at 6 h of 9.6 ± 0.8-fold) and preceded the induction of intestinal Ca absorption (significantly increased at 6 h, peak at 9 h). Neither renal CaT1 nor ECaC mRNA were strongly regulated by dietary calcium level or by 1,25(OH)2 D3 injection. Our data indicate that CaT1 and ECaC mRNA levels are differentially regulated by 1,25(OH)2 D3 in kidney and intestine and that there may be a specialized role for CaT1 in kidney in fetal and neonatal development. The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)2 D3, and the marked induction at weaning, suggests that CaT1 is critical for 1,25(OH)2 D3 mediated intestinal Ca absorption.
Key words: development • calcium • calbindin-D9k • calbindin-D28k calcium absorption • CaT1 • ECaC






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