help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on July 17, 2003
Endocrinology, doi:10.1210/en.2003-0419
A more recent version of this article appeared on October 1, 2003
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
144/10/4459    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Araki, O.
Right arrow Articles by Murakami, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Araki, O.
Right arrow Articles by Murakami, M.

Submitted on April 3, 2003
Accepted on July 7, 2003

Expression of Type 2 Iodothyronine Deiodinase in Corticotropin Secreting Mouse Pituitary Tumor Cells is stimulated by Glucocorticoid and Corticotropin Releasing Hormone

Osamu Araki1, Tadashi Morimura1, Takayuki Ogiwara1, Haruo Mizuma1, Masatomo Mori1, and Masami Murakami1*

1 First Department of Internal Medicine and Department of Laboratory Medicine, Gunma University School of Medicine, Maebashi 371, Japan

* To whom correspondence should be addressed. E-mail: mmurakam{at}showa.gunma-u.ac.jp.

We identified the presence of iodothyronine deiodinase in AtT-20 mouse pituitary tumor cells which secrete corticotropin. Iodothyronine deiodinating activity in AtT-20 cells fulfills all the characteristics of type 2 iodothyronine deiodinase (D2), including the inhibition by thyroid hormones, the insensitivity to inhibition by 6-propyl-2-thiouracil, and the low Km value for T4. Northern analysis using mouse D2 cRNA probe demonstrated the hybridization signal with approximately 7.0 kb in size in AtT-20 cells. D2 activity and D2 mRNA were stimulated by glucocorticoid in a dose dependent manner, but were not stimulated by testosterone or {beta}-estradiol. D2 expression was stimulated by (Bu)2cAMP, and CRH in a dose dependent manner in the presence of dexamethasone. These results suggest the previously unrecognized role of local thyroid hormone activation by D2 in the regulation of pituitary corticotrophs.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2003 by The Endocrine Society