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Submitted on ,
Accepted on ,
1 Department of Zoology & Howard Florey Institute of Experimental Physiology & Medicine, University of Melbourne, Parkville, Victoria, 3010, Australia
* To whom correspondence should be addressed.
This study used relaxin (RLX) gene knockout mice (Rlx-) to investigate the effects of RLX on myometrial oxytocin receptor (OTR) and estrogen receptor-
(ER) gene expression in late gestation. We also characterized the temporal expression of the RLX receptor (LGR7) and demonstrated gene transcripts in the myometrium of Rlx+/+ and Rlx- mice. There was a significant (P < 0.05) decrease in myometrial LGR7 gene expression on days 17.5 and 18.5 pc compared with earlier stages of gestation, but no differences between Rlx+/+ and Rlx- mice. Myometrial OTR mRNA levels increased at the end of gestation in Rlx+/+ but not Rlx- mice. ER gene expression was up-regulated on day 14.5 pc in Rlx+/+ mice, with mRNA levels remaining high throughout late gestation. In contrast, ER mRNA levels were significantly lower in Rlx- mice on days 14.5 and 18.5 pc. These data show that the increases in myometrial OTR and ER expression in late pregnant Rlx+/+ mice were attenuated in Rlx- mice. The effects of RLX on OTRs are probably mediated via activation of ER s. Finally, RLX receptor expression in the myometrium of Rlx- mice did not differ from wild-type mice, implying that RLX does not influence expression of its receptor.
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