| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 10, 2003
Accepted on August 12, 2003
1 Department of Research (P.L., D.S.), Division of Endocrinology, Diabetology and Clinical Nutrition (U.K., B.M.), Department of Visceral Surgery (I.L.), and Department of Plastic Surgery (M.S.), University Hospitals, CH-4031 Basel, Switzerland and Dept. of Medicine, George Washington University and Veterans Affairs Medical Center (E.S.N., K.L.B.), Washington, DC 20422
* To whom correspondence should be addressed. E-mail: Philippe.Linscheid{at}unibas.ch.
Circulating levels of calcitonin precursors (CTpr), including procalcitonin (ProCT), increase up to several-thousand-fold in human sepsis and immunoneutralization improves survival in two animal models of this disease. Herein, we analyzed inflammation-mediated calcitonin-I gene (CALC I) gene expression in human adipocyte primary cultures and in adipose tissue samples from infected and non-infected patients with different levels of serum ProCT. In ex vivo differentiated adipocytes, expression of CT-mRNA increased 24-fold (P < 0.05) by the administration of Escherichia coli endotoxin (LPS) and 37-fold (P < 0.05) by interleukin-1
(IL-1) after 6 h. ProCT protein secretion into culture supernatant increased 13.5-fold (P < 0.01) with LPS treatment and 15.2-fold (P < 0.01) by IL-1 after 48 h. In co-culture experiments, adipocyte CT-mRNA expression was evoked by Escherichia coli activated macrophages, in which CT-mRNA was undetectable. The marked IL-1-mediated-ProCT release was inhibited by 89% during co-administration with interferon-
(IFN-). In patients with infection and markedly increased serum ProCT, CT-mRNA was detected in adipose tissue biopsies. Hence, we demonstrate that ProCT, which is suspected to mediate deleterious effects in sepsis and inflammation, is a novel product of adipose tissue secretion. The inhibiting effect of IFN- on IL-1 -induced CT-mRNA expression and on ProCT secretion might explain previous observations that serum ProCT concentrations increase less in systemic viral compared with bacterial infections.
•
interferon- •
endotoxin
This article has been cited by other articles:
 |
|
 |
|
  M. Christ-Crain and B. Muller Biomarkers in respiratory tract infections: diagnostic guides to antibiotic prescription, prognostic markers and mediators Eur. Respir. J., September 1, 2007; 30(3): 556 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Desruisseaux, Nagajyothi, M. E. Trujillo, H. B. Tanowitz, and P. E. Scherer Adipocyte, Adipose Tissue, and Infectious Disease Infect. Immun., March 1, 2007; 75(3): 1066 - 1078. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Christ-Crain, D. Stolz, R. Bingisser, C. Muller, D. Miedinger, P. R. Huber, W. Zimmerli, S. Harbarth, M. Tamm, and B. Muller Procalcitonin Guidance of Antibiotic Therapy in Community-acquired Pneumonia: A Randomized Trial Am. J. Respir. Crit. Care Med., July 1, 2006; 174(1): 84 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Linscheid, D. Seboek, H. Zulewski, A. Scherberich, N. Blau, U. Keller, and B. Muller Cytokine-induced metabolic effects in human adipocytes are independent of endogenous nitric oxide Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1068 - E1077. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Puder, S. Varga, M. Kraenzlin, C. De Geyter, U. Keller, and B. Muller Central Fat Excess in Polycystic Ovary Syndrome: Relation to Low-Grade Inflammation and Insulin Resistance J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6014 - 6021. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Linscheid, D. Seboek, H. Zulewski, U. Keller, and B. Muller Autocrine/Paracrine Role of Inflammation-Mediated Calcitonin Gene-Related Peptide and Adrenomedullin Expression in Human Adipose Tissue Endocrinology, June 1, 2005; 146(6): 2699 - 2708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Muller Procalcitonin and Ventilator-associated Pneumonia: Yet Another Breath of Fresh Air Am. J. Respir. Crit. Care Med., January 1, 2005; 171(1): 2 - 3. [Full Text] [PDF]
|
|
|
|
|
|
D. Seboek, P. Linscheid, H. Zulewski, I. Langer, M. Christ-Crain, U. Keller, and B. Muller Somatostatin Is Expressed and Secreted by Human Adipose Tissue upon Infection and Inflammation J. Clin. Endocrinol. Metab., October 1, 2004; 89(10): 4833 - 4839. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Becker, E. S. Nylen, J. C. White, B. Muller, and R. H. Snider Jr. Procalcitonin and the Calcitonin Gene Family of Peptides in Inflammation, Infection, and Sepsis: A Journey from Calcitonin Back to Its Precursors J. Clin. Endocrinol. Metab., April 1, 2004; 89(4): 1512 - 1525. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
