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Submitted on November 26, 2003
Accepted on January 8, 2004
From: (1) The Department of Obstetrics, Gynecology and Reproductive Science and (2) Genetics Yale University, School of Medicine. New Haven, CT.
* To whom correspondence should be addressed. E-mail: graciela.krikun{at}yale.edu.
Obtaining primary human endometrial stromal cells (HESCs) for in vitro studies is limited by the scarcity of adequate human material and the inability to passage these cells in culture for long periods. Immortalization of these cells would greatly facilitate studies; however the process of immortalization often results in abnormal karyotypes and aberrant functional characteristics. To meet this need, we have introduced telomerase into cultured HESCs to prevent the normal shortening of telomeres observed in adult somatic cells during mitosis. We have now developed and analyzed a newly immortalized HESCs cell line which contains no clonal chromosomal structural or numerical abnormalities. In addition, when compared with the primary unpassaged parent cells, the new cell line displayed similar biochemical endpoints following treatment with ovarian steroids. Classical decidualization response to estradiol (E2) + medroxyprogesterone acetate (MPA) were seen both morphologically and progestin was seen to induce or regulate the expression of insulin-like growth factor binding protein-1 (IGFBP-1), fibronectin (FN), prolactin (PRL), tissue factor (TF), plasminogen activator inhibitor-1 (PAI-1) and Fas/Fas ligand. In summary, an immortalized endometrial stromal cell line has been developed which is karyotypically, morphologically and phenotypically similar to the primary parent cells and are a powerful and consistent resource for in vitro work
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