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This version published online on June 17, 2004
Endocrinology, doi:10.1210/en.2004-0589
A more recent version of this article appeared on September 1, 2004
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Submitted on May 10, 2004
Accepted on June 7, 2004

Expression of Vesicular Glutamate Transporter-2 in Gonadotropin-Releasing Hormone Neurons of the Adult Male Rat

ERIK HRABOVSZKY*, GERGELY F. TURI, IMRE KALLÓ, and ZSOLT LIPOSITS

Laboratory of Endocrine Neurobiology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083 Hungary

* To whom correspondence should be addressed. E-mail: hrabovszky{at}koki.hu.

Isoforms of the recently cloned vesicular glutamate transporters (VGLUT1-3) selectively accumulate glutamic acid into synaptic vesicles in excitatory axon terminals and are viewed as reliable markers for glutamatergic neurons. Our present studies provided dual-label in situ hybridization evidence that virtually all (99.5%) of gonadotropin-releasing hormone (GnRH) neurons express VGLUT2 mRNA in the preoptic region of the adult male rat. Dual-label immunofluorescent experiments were carried out to examine the presence of VGLUT2 protein in GnRH axon terminals. Confocal laser microscopic analysis of the organum vasculosum of the lamina terminalis and the external zone of the median eminence, the major termination fields for GnRH-secreting axons, demonstrated the frequent occurrence of VGLUT2 immunoreactivity in GnRH axon terminals. Together these mRNA hybridization and immunocytochemical data indicate that GnRH neurons of the adult male rat possess marked glutamatergic characteristics. The physiological significance of endogenous glutamate in the regulation of gonadotropin secretion requires clarification.
Key words: EXCITATORY AMINO ACID • GABA • GLUTAMIC ACID • IMMUNOCYTOCHEMISTRY • IN SITU HYBRIDIZATION • LHRH




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