| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 1, 2004
Accepted on September 9, 2004
Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Kitakyushu 807-8555, Japan; U-583 INSERM, L'Institut des Neurosciences de Montpellier, Hopital St Eloi, Montpellier F-34091 cedex 5, France; Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan; Department of Biological Information Processing, Graduate School of Electronic Science and Technology, Shizuoka University, Hamamatsu 432-8011, Japan; Department of Physiology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan; Molecular Neuroendocrinology Research Group, The Henry Wellcome Laboratories for Integrative Neuroscience and Endocrinology, University of Bristol, Dorothy Hodgkin Building, Bristol BS1 3NY, UK
* To whom correspondence should be addressed. E-mail: yoichi{at}med.uoeh-u.ac.jp.
We have generated transgenic rats expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene. The expression of the eGFP gene and strong fluorescence were observed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN) and the suprachiasmatic nucleus (SCN) in transgenic rats. The hypothalamo-neurohypophyseal tract, isolated SON neurons and isolated axon terminals in the neurohypophysis also showed robust eGFP fluorescence. Water deprivation for 2 days increased the fluorescence of the eGFP in the SON and the PVN but not the SCN. The whole-cell patch-clamp technique was then used to record the electrical activities specifically identified eGFP-expressing SON, PVN and SCN AVP neurons in in vitro brain slice preparations. The AVP-eGFP transgenic rats are a unique new tool with which to study the physiological role of AVP-secreting neurons in the central nervous system and the dynamics of the regulation of AVP secretion in the living neurons and their axon terminals.
This article has been cited by other articles:
 |
|
 |
|
  V. A. Tobin, P. M. Bull, S. Arunachalam, A.-M. O'Carroll, Y. Ueta, and M. Ludwig The Effects of Apelin on the Electrical Activity of Hypothalamic Magnocellular Vasopressin and Oxytocin Neurons and Somatodendritic Peptide Release Endocrinology, December 1, 2008; 149(12): 6136 - 6145. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. J. Iremonger and J. S. Bains Integration of Asynchronously Released Quanta Prolongs the Postsynaptic Spike Window J. Neurosci., June 20, 2007; 27(25): 6684 - 6691. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. E. Stachniak and C. W. Bourque Visually guided whole cell patch clamp of mouse supraoptic nucleus neurons in cultured and acute conditions Am J Physiol Regulatory Integrative Comp Physiol, July 1, 2006; 291(1): R68 - R76. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
