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Submitted on July 27, 2004
Accepted on December 13, 2004
Department of Human and Animal Biology, University of Torino, Via Accademia Albertina 13, 10123, Torino, Italy
* To whom correspondence should be addressed. E-mail: isabelle.perroteau{at}unito.it.
Expression of stathmin, a microtubule-associated cytoplasmic protein, prominently localized in neuroproliferative zones and neuronal migration pathways in brain, was investigated in the GnRH neuroendocrine system in vivo and the function was analyzed using an in vitro approach.
Here we present novel data demonstrating that GnRH migrating neurons in nasal regions and basal forebrain areas of mouse embryos express stathmin protein. In addition, this expression pattern is dependent on location, as GnRH neurons reaching the hypothalamus are stathmin-negative. Immortalized GN-11 cells, which retain many characteristics of migrating GnRH neurons, strongly express stathmin mRNA and protein. The role of stathmin in GnRH migratory properties was evaluated using GN-11 cell line. We up- (STMN+) and down-regulated (STMN-), the expression of stathmin in GN-11 cells and we investigated changes in cell morphology and motility in vitro. Cells over-expressing stathmin assume a spindle-shaped morphology and their proliferation, as well as their motility, is higher with respect to parental cells. Furthermore, they do not aggregate and express low levels of cadherins compared with control cells. STMN- GN-11 cells, are endowed with multipolar processes, they show a decreased motility and express high levels of cadherin protein. Our findings suggest that stathmin plays a permissive role in GnRH cell motility, possibly via modulation of cadherins expression.
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