| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 30, 2004
Accepted on September 24, 2004
Department of Veterinary Preclinical Studies, University of Glasgow Veterinary School, University of Glasgow, Bearsden Road, Glasgow, G61 1QH, United Kingdom
* To whom correspondence should be addressed. E-mail: p.j.o'shaughnessy{at}vet.gla.ac.uk.
Changes in gene expression during granulosa cell luteinization have been measured using serial analysis of gene expression (SAGE). Immature normal mice were treated with pregnant mare serum gonadotrophin (PMSG) or PMSG followed, 48 h later, by human chorionic gonadotrophin (hCG). Granulosa cells were collected from pre-ovulatory follicles after PMSG injection or PMSG/hCG injection and SAGE libraries generated from the isolated mRNA. The combined libraries contained 105,224 tags representing 40,248 unique transcripts. Overall, 715 transcripts showed a significant difference in abundance between the two libraries of which 216 were significantly down-regulated by hCG and 499 were significantly up-regulated. Among transcripts differentially regulated there were clear and expected changes in genes involved in steroidogenesis as well as clusters of genes involved in modeling of the extracellular matrix, regulation of the cytoskeleton and intra and inter-cellular signaling. The SAGE libraries described here provide a base for functional investigation of the regulation of granulosa cell luteinization.
This article has been cited by other articles:
 |
|
 |
|
  M. Hamel, I. Dufort, C. Robert, C. Gravel, M.-C. Leveille, A. Leader, and M.-A. Sirard Identification of differentially expressed markers in human follicular cells associated with competent oocytes Hum. Reprod., May 1, 2008; 23(5): 1118 - 1127. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Mihm, P J Baker, L M Fleming, A M Monteiro, and P J O'Shaughnessy Differentiation of the bovine dominant follicle from the cohort upregulates mRNA expression for new tissue development genes Reproduction, February 1, 2008; 135(2): 253 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Q. Su, M. Nyegaard, M. T. Overgaard, J. Qiao, and L. C. Giudice Participation of Mitogen-Activated Protein Kinase in Luteinizing Hormone-Induced Differential Regulation of Steroidogenesis and Steroidogenic Gene Expression in Mural and Cumulus Granulosa Cells of Mouse Preovulatory Follicles Biol Reprod, December 1, 2006; 75(6): 859 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Rodriguez, L. A. Blomberg, K. A. Zuelke, J. R. Miles, J. E. Alexander, and C. E. Farin Identification of candidate mRNAs associated with gonadotropin-induced maturation of murine cumulus oocyte complexes using serial analysis of gene expression Physiol Genomics, November 21, 2006; 27(3): 318 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Agca, J. E Ries, S. J Kolath, J.-H. Kim, L. J Forrester, E. Antoniou, K. M Whitworth, N. Mathialagan, G. K Springer, R. S Prather, et al. Luteinization of porcine preovulatory follicles leads to systematic changes in follicular gene expression Reproduction, July 1, 2006; 132(1): 133 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Peluso Multiplicity of Progesterone's Actions and Receptors in the Mammalian Ovary Biol Reprod, July 1, 2006; 75(1): 2 - 8. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hernandez-Gonzalez, I. Gonzalez-Robayna, M. Shimada, C. M. Wayne, S. A. Ochsner, L. White, and J. S. Richards Gene Expression Profiles of Cumulus Cell Oocyte Complexes during Ovulation Reveal Cumulus Cells Express Neuronal and Immune-Related Genes: Does this Expand Their Role in the Ovulation Process? Mol. Endocrinol., June 1, 2006; 20(6): 1300 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Peluso, A. Pappalardo, R. Losel, and M. Wehling Progesterone Membrane Receptor Component 1 Expression in the Immature Rat Ovary and Its Role in Mediating Progesterone's Antiapoptotic Action Endocrinology, June 1, 2006; 147(6): 3133 - 3140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-H. Ma, S.-J. Hu, H. Ni, Y.-C. Zhao, Z. Tian, J.-L. Liu, G. Ren, X.-H. Liang, H. Yu, P. Wan, et al. Serial Analysis of Gene Expression in Mouse Uterus at the Implantation Site J. Biol. Chem., April 7, 2006; 281(14): 9351 - 9360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. A. Tullet, V. Pocock, J. H. Steel, R. White, S. Milligan, and M. G. Parker Multiple Signaling Defects in the Absence of RIP140 Impair Both Cumulus Expansion and Follicle Rupture Endocrinology, September 1, 2005; 146(9): 4127 - 4137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. Couse, M. M. Yates, B. J. Deroo, and K. S. Korach Estrogen Receptor-{beta} Is Critical to Granulosa Cell Differentiation and the Ovulatory Response to Gonadotropins Endocrinology, August 1, 2005; 146(8): 3247 - 3262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Peluso, A. Pappalardo, R. Losel, and M. Wehling Expression and Function of PAIRBP1 Within Gonadotropin-Primed Immature Rat Ovaries: PAIRBP1 Regulation of Granulosa and Luteal Cell Viability Biol Reprod, August 1, 2005; 73(2): 261 - 270. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
