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Submitted on January 18, 2005
Accepted on April 19, 2005
Biocenter Oulu and Department of Anatomy and Cell Biology (P.M.A., U.E.P.-R.) and Department of Anatomy and Cell Biology (J.T.A, H.J.R), University of Oulu, FI-90014, Oulu, Finland
* To whom correspondence should be addressed. E-mail: Ulla.Petaja-Repo{at}oulu.fi.
The LH receptor (LHR) is a G protein-coupled receptor involved in regulation of ovarian and testicular functions. Here we demonstrate novel and unexpected patterns of receptor expression and regulation in fetal and adult rodent urogenital and adrenal tissues. Two rat LHR promoter fragments (
2 kb and 4 kb) were shown to direct expression of the lacZ reporter in transgenic mice to gonads, adrenal glands and kidneys, starting at 14.5 days post coitum (dpc), and to genital tubercles, starting at 11.5 dpc. These tissues were also found to express the full-length LHR mRNA and protein during rat fetal development but, importantly, only immature receptors carrying unprocessed N-linked glycans were detected. After birth the receptor gene activity ceased, except in the gonads, which started to express the mature receptor carrying fully processed N-linked glycans. Surprisingly, both LHR mRNA and mature protein levels were upregulated substantially in pregnant female adrenal glands and kidneys, at a time that coincides with differentiation of the fetal urogenital tissues. Taken together, these results indicate that the LHR protein is expressed constitutively in the gonadal and non-gonadal urogenital tissues as well in the adrenal glands but its final functional maturation at post-translational level appears to be developmentally and physiologically regulated.
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