| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on July 13, 2005
Accepted on October 3, 2005
Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland, Department of Physiology, University of Turku, 20520 Turku, Finland, Graduate School of Integrated Science and Art, University of East Asia, Yamaguchi 750-8503, Japan
* To whom correspondence should be addressed. E-mail: ziecik{at}pan.olsztyn.pl.
Endometrial prostaglandins (PGs) and the PGE2/PGF2
ratio play an important role in regulating the estrous cycle and establishment of pregnancy. The enzymes downstream of cyclooxygenase-2 may determine the PGE2/PGF2 ratio in the porcine uterus. Thus, we have cloned porcine PGF synthase (PGFS) and microsomal PGE synthase-1 (mPGES-1) and characterized their expression in porcine endometrium during the estrous cycle and early pregnancy. PGFS and mPGES-1 amino acid sequences possessed a high degree (>67% and >77%, respectively) of identity with the other mammalian homologs. There was little modulation of mPGES-1 throughout the estrous cycle; however, PGFS expression was highly upregulated in endometrium around the time of luteolysis. During early pregnancy, PGFS at the protein level showed a time-dependent increase (low on days 10-13, intermediate on days 14-23, and high on days 24-25). In pregnancy, expression of mPGES-1 was intermediate on days 10-11, low on days 14-17 and then increased after day 22, reaching the maximum on days 24-25. Immunohistochemistry showed localization of PGFS and mPGES-1 proteins mainly in luminar and glandular epithelium. Concluding, the spatio-temporal expression of PGFS throughout the estrous cycle indicates an involvement of PGFS in regulating luteolysis in the pig. The comparison of endometrial PGFS and mPGES-1 expression on days 10-13 of the estrous cycle and pregnancy suggest a supportive role of these enzymes in determining the increase of uterine PGE2/PGF2 ratio during maternal recognition of pregnancy. However, high expression of both PG synthases after initiation of implantation may indicate their significant role in placentation.
This article has been cited by other articles:
 |
|
 |
|
  A. Waclawik and A. J Ziecik Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig J. Endocrinol., September 1, 2007; 194(3): 499 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Samuelsson, R. Morgenstern, and P.-J. Jakobsson Membrane Prostaglandin E Synthase-1: A Novel Therapeutic Target Pharmacol. Rev., September 1, 2007; 59(3): 207 - 224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wada, C. J. DeLong, Y. H. Hong, C. J. Rieke, I. Song, R. S. Sidhu, C. Yuan, M. Warnock, A. H. Schmaier, C. Yokoyama, et al. Enzymes and Receptors of Prostaglandin Pathways with Arachidonic Acid-derived Versus Eicosapentaenoic Acid-derived Substrates and Products J. Biol. Chem., August 3, 2007; 282(31): 22254 - 22266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Mueller, T. Maltaris, J. Siemer, H. Binder, I. Hoffmann, M. W. Beckmann, and R. Dittrich Uterine contractility in response to different prostaglandins: results from extracorporeally perfused non-pregnant swine uteri Hum. Reprod., August 1, 2006; 21(8): 2000 - 2005. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
