help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on March 9, 2006
Endocrinology, doi:10.1210/en.2005-1130
A more recent version of this article appeared on June 1, 2006
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
147/6/3085    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ng, H P
Right arrow Articles by Kung, A. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ng, H P
Right arrow Articles by Kung, A. W.

Submitted on September 2, 2005
Accepted on February 27, 2006

Induction of Autoimmune Thyroiditis and Hypothyroidism by Immunization of Immunoactive T Cell Epitope of Thyroid Peroxidase

H P Ng and Annie WC Kung*

Department of Medicine, The University of Hong Kong, Pokfulam, Hong Kong, China

* To whom correspondence should be addressed. E-mail: awckung{at}hkucc.hku.hk.

Autoimmune thyroiditis (AT) is characterized by a continuous inflammatory self-destructive process that eventually leads to chronic progressive dysfunction of the thyroid. In a previously established experimental autoimmune thyroiditis (EAT) model, C57bl/6 mice immunized with recombinant mouse thyroid peroxidase (rmTPO) developed lymphocytic thyroiditis and anti-TPO antibody but not chronic hypothyroidism. To determine the immunodominant epitope(s) of TPO, T cell proliferation assays were performed in which rmTPO-primed lymph nodes cells were reacted with recombinant mTPO fragments or short overlapping synthetic TPO peptides. Within residues 405 to 849, peptide 540-559 gave the maximum proliferation response with a stimulation index >12. Mice immunized with peptide 540-559 developed antibody against rmTPO and native mouse TPO protein, lymphocytic thyroiditis and hypothyroidism. In conclusion, this study demonstrated that TPO is the autoantigen for the development of lymphocyte thyroiditis and thyroid dysfunction, and peptide 540-559 is the immunodominant T cell epitope of TPO. Identification of T cell epitopes of TPO may enable the development of immunotherapy to prevent chronic hypothyroidism in AT.
Key words: Thyroid peroxidase • Autoimmune Thyroiditis • murine model




This article has been cited by other articles:


Home page
 EndocrinologyHome page
S. M. McLachlan, Y. Nagayama, P. N. Pichurin, Y. Mizutori, C.-R. Chen, A. Misharin, H. A. Aliesky, and B. Rapoport
The Link between Graves' Disease and Hashimoto's Thyroiditis: A Role for Regulatory T Cells
Endocrinology, December 1, 2007; 148(12): 5724 - 5733.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Dubska, J. P. Banga, D. Plochocka, G. Hoser, E. H. Kemp, B. J. Sutton, A. Gardas, and M. Gora
Structural Insights into Autoreactive Determinants in Thyroid Peroxidase Composed of Discontinuous and Multiple Key Contact Amino Acid Residues Contributing to Epitopes Recognized by Patients' Autoantibodies
Endocrinology, December 1, 2006; 147(12): 5995 - 6003.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2006 by The Endocrine Society