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Submitted on December 9, 2005
Accepted on January 17, 2006
Institut National de la Recherche Agronomique, INRA - SCRIBE, IFR 140, Campus Beaulieu 35000 Rennes, France; Department of Fisheries Sciences, Faculty of Agriculture and Natural Resources, University of Kurdestan, 416, Sanandaj, Iran
* To whom correspondence should be addressed. E-mail: Julien.Bobe{at}rennes.inra.fr.
In the present study, we report the full coding sequence of rainbow trout IGFBP 1, 2, 3, 5, 6 and IGFBP-related protein 1 (IGFBP-rP1) mRNAs as well as the partial coding sequence of IGFBP4 mRNA. To our knowledge, this is the first report of IGFBP4, 6 and IGFBP-rP1 in a non mammalian species. The tissue distribution of all mRNAs was studied and the ovarian expression profiles of IGFBP2-6 and IGFBP-rP1 between late vitellogenesis and oocyte maturation were characterized. In addition, in vitro hormonal regulation by the maturation-inducing steroid (17,20
-dihydroxy-4pregnen-3one, 17,20P), gonadotropin and estradiol (E2) were studies. We observed that besides IGFBP1, that was only found in liver, IGFBP2-6 and IGFPB-rP1 were expressed in the preovulatory ovary. IGFBP3 was also detected in liver, trunk kidney, skin, gills while IGFBP2, 4, 5, 6 and IGFBP-rP1 exhibited a wider tissue distribution. In the preovulatory ovary, IGFBP3 was strongly down-regulated during post-vitellogenesis while IGFBP5 exhibited a limited up-regulation. In addition, IGFBP6 and IGFBP-rP1 were up-regulated during oocyte maturation. Hormonal regulation data indicated that all ovarian IGFBPs and IGFBP-rP1 transcripts are regulated under gonadotropic stimulation, at a concentration that induced 100% oocyte maturation. In addition, IGFBP2-5 transcripts are also regulated by 17,20P and E2. Together, our observations strongly suggest that, during final oocyte maturation, a down-regulation of IGFBP3, 4 and 5 occurs in the oocyte in response to gonadotropic and 17,20P (IGFBP3 and 5) stimulation, while an up-regulation of IGFBP2 and 6 occurs in follicular layers or extrafollicular tissue in response to gonadotropic stimulation.
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