help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on May 18, 2006
Endocrinology, doi:10.1210/en.2006-0417
A more recent version of this article appeared on October 1, 2006
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
147/10/4553    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by den Boer, M. A.M.
Right arrow Articles by Romijn, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by den Boer, M. A.M.
Right arrow Articles by Romijn, J. A.

Submitted on March 31, 2006
Accepted on May 10, 2006

Endogenous IL-10 Protects Against Hepatic Steatosis, but Does Not Improve Insulin Sensitivity During High Fat Feeding in Mice

Marion A.M. den Boer*, Peter J. Voshol, Janny P. Schröder-van der Elst, Elena Korsheninnikova, D. Margriet Ouwens, Folkert Kuipers, Louis M. Havekes, and Johannes A. Romijn

Dept. of Endocrinology and Metabolism, LUMC, Dept. of Molecular Cell Biology, LUMC, Leiden, Dept. of Pediatrics, University Medical Center Groningen, Groningen, Dept. of Cardiology, Leiden University Medical Center, Leiden, Netherlands; TNO Quality of Life, Leiden, Netherlands

* To whom correspondence should be addressed. E-mail: A.M.den Boer{at}lumc.nl.

Several studies have demonstrated an association in humans between plasma levels or production capacity of the anti-inflammatory cytokine IL-10, and insulin sensitivity. The aim of our study was to investigate the protective role of endogenous IL-10 availability in the development of diet-induced insulin resistance. We compared parameters of glucose and lipid metabolism between IL-10-/- mice and wild-type (wt) mice fed a high fat diet for 6 weeks. This diet has previously been shown to induce steatosis and insulin resistance. After 6 weeks on the high fat diet no differences in bodyweight, basal metabolism (measured by indirect calorimetry) and plasma levels of glucose, triglycerides (TG) or cholesterol were observed between IL-10-/- and wt mice. Nonetheless, in IL-10-/- mice plasma free fatty acid levels were 75% increased compared with wt mice after overnight fasting (P < 0.05). In addition, hepatic TG content was 54% increased in IL-10-/- mice (P < 0.05). During a hyperinsulinemic euglycemic clamp no differences were observed in whole-body or hepatic insulin sensitivity between both groups.

We conclude that basal IL-10 production protects against hepatic steatosis, but does not improve hepatic or whole-body insulin sensitivity, during high fat feeding.
Key words: Free fatty acid • Insulin resistance • Cytokine • Inflammation • Hyperinsulinemic euglycemic clamp technique




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
Y. Wei, K. Chen, A. T. Whaley-Connell, C. S. Stump, J. A. Ibdah, and J. R. Sowers
Skeletal muscle insulin resistance: role of inflammatory cytokines and reactive oxygen species
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R673 - R680.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
R. Barbuio, M. Milanski, M. B Bertolo, M. J Saad, and L. A Velloso
Infliximab reverses steatosis and improves insulin signal transduction in liver of rats fed a high-fat diet
J. Endocrinol., September 1, 2007; 194(3): 539 - 550.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Peters
The energy request of inflammation.
Endocrinology, October 1, 2006; 147(10): 4550 - 4552.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2006 by The Endocrine Society