help button home button Endocrine Society Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
This version published online on November 22, 2006
Endocrinology, doi:10.1210/en.2006-1003
A more recent version of this article appeared on March 1, 2007
This Article
Right arrow Author Manuscript (PDF)
Right arrow All Versions of this Article:
148/3/1108    most recent
Author Manuscript (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Faenza, I.
Right arrow Articles by Cocco, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Faenza, I.
Right arrow Articles by Cocco, L.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

Submitted on July 26, 2006
Accepted on November 13, 2006

Inositide-dependent phospholipase C signaling mimics insulin in skeletal muscle differentiation by affecting specific regions of the cyclin D3 promoter

Irene Faenza, Giulia Ramazzotti, Alberto Bavelloni, Roberta Fiume, Gian Carlo Gaboardi, Matilde Y. Follo, R. Stewart Gilmour, Alberto M. Martelli, Katya Ravid, and Lucio Cocco*

Cellular Signalling Laboratory, Department of Human Anatomical Science, University of Bologna, via Irnerio 48, 40126 Bologna, Italy; Laboratory of Cell Biology and Electron Microscopy, IOR, Bologna Italy; Department of Pathology, University of Cambridge, Cambridge CB2 1QP, U.K.; Department of Biochemistry, Department of Medicine, and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, Massachusetts 02118

* To whom correspondence should be addressed. E-mail: lcocco{at}biocfarm.unibo.it.

Our main goal in this study was to investigate the role of phospholipase C (PLC) {beta}1 and PLC{gamma}1 in skeletal muscle differentiation and the existence of potential downstream targets of their signaling activity. To examine whether PLC signaling can modulate the expression of cyclin D3, a target of PLC{beta}1 in erythroleukemia cells, we transfected C2C12 cells with expression vectors containing PLC{beta}1 or PLC{gamma}1 cDNA and with small interfering RNAs (siRNAs) from regions of the PLC{beta}1 or PLC{gamma}1 gene and followed myogenic differentiation in this well-established cell system. Intriguingly, overexpressed PLC{beta}1 and PLC{gamma}1 were able to mimic insulin induction of both cyclin D3 and muscle differentiation. By knocking down PLC{beta}1 or PLC{gamma}1 expression, C2C12 cells almost completely lost the increase in cyclin D3, and the differentiation program was down-regulated. To explore the induction of the cyclin D3 gene promoter during this process, we used a series of 5'-deletions of the 1.68 kb promoter linked to a reporter gene and noted a 5-fold augmentation of promoter activity upon insulin stimulation. These constructs were also co-transfected with PLC{beta}1 or PLC{gamma}1 cDNAs and siRNAs, respectively. Our data indicate that PLC{beta}1 or PLC{gamma}1 signaling is capable of acting like insulin in regard to both the myogenic differentiation program and cyclin D3 up-regulation. Taken together, this is the first study which hints at cyclin D3 as a target of PLC{beta}1 and PLC{gamma}1 during myogenic differentiation in vitro and implies that up-regulation of these enzymes is sufficient to mimic the actions of insulin in this process.
Key words: phospholipase C • cyclin D3 • nucleus • insulin • myogenic differentiation




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
Y. Ding, K. J. Choi, J. H. Kim, X. Han, Y. Piao, J.-H. Jeong, W. Choe, I. Kang, J. Ha, H. J. Forman, et al.
Endogenous Hydrogen Peroxide Regulates Glutathione Redox via Nuclear Factor Erythroid 2-Related Factor 2 Downstream of Phosphatidylinositol 3-Kinase during Muscle Differentiation
Am. J. Pathol., June 1, 2008; 172(6): 1529 - 1541.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
F. De Santa, S. Albini, E. Mezzaroma, L. Baron, A. Felsani, and M. Caruso
pRb-Dependent Cyclin D3 Protein Stabilization Is Required for Myogenic Differentiation
Mol. Cell. Biol., October 15, 2007; 27(20): 7248 - 7265.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. Evangelisti, P. L. Tazzari, M. Riccio, R. Fiume, Y. Hozumi, F. Fala, K. Goto, L. Manzoli, L. Cocco, and A. M. Martelli
Nuclear diacylglycerol kinase-{zeta} is a negative regulator of cell cycle progression in C2C12 mouse myoblasts
FASEB J, October 1, 2007; 21(12): 3297 - 3307.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. J. Lim, K. J. Choi, Y. Ding, J. H. Kim, B. S. Kim, Y. H. Kim, J. Lee, W. Choe, I. Kang, J. Ha, et al.
RhoA/Rho Kinase Blocks Muscle Differentiation via Serine Phosphorylation of Insulin Receptor Substrate-1 and -2
Mol. Endocrinol., September 1, 2007; 21(9): 2282 - 2293.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2006 by The Endocrine Society