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Submitted on June 1, 2007
Accepted on July 10, 2007
Departments of OB/GYNand Cellular and Integrative Physiology, Durham Research Center, University of Nebraska Medical Center, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center
* To whom correspondence should be addressed. E-mail: skroy{at}unmc.edu.
The non-genomic actions of estradiol-17
(E2) are mediated by transmembrane estrogen receptors (mERs). Recently, G-protein coupled-receptor 30 (GPR30) has been suggested to be a mER that can mediate rapid and transcription-independent E2 signaling in different cell types. However, the expression, regulation or biological relevance of GPR30 in the ovary remains unknown. We examined the expression and hormonal regulation of GPR30 mRNA and protein in hamster ovarian cells during the estrous cycle, and following hypophysectomy and hormone replacement. GPR30 protein expression was high in the theca, appreciable in the granulosa, but low in luteal cells. GPR30 protein levels in granulosa and theca cells increased steadily with the development of preantral and antral follicles, respectively. GPR30 mRNA and protein levels increased significantly on diestrous (day 3 of the estrous cycle), but decreased at day 4:1600h following the LH surge. GPR30 mRNA levels increased significantly following hypophysectomy (HX). Whereas steroid treatment failed to alter ovarian GPR30 mRNA levels, either FSH or LH effectively reduced the levels. Interestingly, the decreased in GPR30 mRNA corresponded to a marked increase in the receptor protein levels. FSH treatment, either alone or together with LH, resulted in a marked increase in GPR30 immunostaining in granulosa cells. LH alone significantly increased immunostaining in theca cells. These results suggest that GPR30 is expressed in the membrane of hamster granulosa and theca cells, and the expression is regulated by gonadotropins. The unique pattern of GPR30 expression suggests that gonadotropin regulated follicular cell functions may involve GPR30 activity.
•
Progesterone •
Gonadotropins
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