Kisspeptins are extraordinarily potent in stimulating gonadotropic hormone secretion via an action on the hypothalamic gonadotropin-releasing hormone (GnRH) neural system. Since the physiologic frequency of the GnRH pulse generator is a critical component of the control system that governs reproductive processes, the aim of this study was to examine the effect of kisspeptin-10 on pulsatile LH secretion and on the electrophysiological manifestation of GnRH pulse generator activity in order to determine frequency modulatory effects. Adult Sprague Dawley rats were ovariectomised and chronically implanted with electrodes in the arcuate nucleus to record the characteristic increases in hypothalamic multiunit electrical activity (MUA volleys) coincident with the initiation of each luteinizing hormone (LH) pulse measured in peripheral blood, and/or indwelling cardiac catheters for the collection of blood samples (25 µl) every 5 min for 6–7 h for the measurement of LH. Intravenous infusion of kisspeptin-10 (7.5, 35 and 100 nmol) induced a dose-dependent increase in LH secretion. The stimulatory effect of kisspeptin-10 (100nmol) on LH secretion was blocked by the GnRH antagonist Cetrorelix, precluding a singular action on gonadotropes. Unexpectedly, however, the marked increase in LH release in response to kisspeptin-10 (100 nmol) administration was not accompanied by any change in MUA volley frequency. It seem unlikely, therefore, that kisspeptin-10 has an appreciable frequency modulatory effect on GnRH pulse generator activity in the female rat.